Tal­mu­dic Ques­tion #101

How many cells does it take to make a biofilm?

 

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8 Comments
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Ami Bachar
13 years ago

I would say a cou­ple of thou­sands. My rea­son is the DBL — the dif­fu­sive bound­ary layer — since it takes sev­eral thou­sands of cells of bac­te­ria to form enough sur­face area that would be af­fect­ing sig­nif­i­cant mass trans­fer of chem­i­cal species.
This is a chicken and egg ques­tion now, since you could al­ways ask me: what is "sig­nif­i­cant" mass trans­fer? good ques­tion, I would an­swer. I think it de­pends on the eco­log­i­cal niche. if it is the sea, than a biofilm of 1 cm^2 do noth­ing to the oceans mass trans­fer. If it is a small flow cell than... well you get what Im say­ing

Matt
13 years ago

2 cells: one cell to at­tach to a sur­face and the other to at­tach to the first cell.
Or per­haps a sin­gle cell biofilm; just a cell at­tached to a sur­face pro­duc­ing LPS.

Maggie Evans
13 years ago

It only takes one cell to cre­ate a biofilm. For in­stance, colony biofilms arise from a sin­gle cell on a plate. Or is the ques­tion how many cells have to be present to be con­sid­ered a biofilm? A biofilm is de­fined as a com­mu­nity of bac­te­ria (as well as eDNA, pro­teins, ex­opolysac­cha­rides etc.). So how many cells have to be present to be con­sid­ered a com­mu­nity?

Christoph Weigel
13 years ago

"It takes two to party" doesn't hold for bugs, i fear. In the known cases the biofilm party only gets started if enough planc­tonic bugs around crave for party (quo­rum sens­ing) loud enough. So it takes prob­a­bly more the equiv­a­lent to a well pop­u­lated disco...

Daniel
13 years ago

None. As­sum­ing a broader de­f­i­n­i­tion of life, a self repli­cat­ing biopoly­mer, such as a ri­bozyme, with an affin­ity for a nearby sur­face would suf­fice.

EL
13 years ago

Ac­cord­ing to Tal­mu­dic law it would be ten adult bac­te­ria. . .

Doug Cossar
13 years ago

Does one bac­terium a pop­u­la­tion make? If 'yes' then one mi­crobe can be a biofilm. If 'No' then two bac­te­ria can be a biofilm (with the caveat that the two must be within 'com­mu­ni­ca­ble' dis­tance of each other). Pre­sum­ing a biofilm rep­re­sents a vi­able pop­u­la­tion of mi­crobes on a sur­face which re­sist re­moval.

bks
13 years ago

Same as the num­ber of neu­rons it takes to make a brain.
–bks